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Simpson, Garth J.; Cheng, Ji-Xin; Min, Wei (Ed.)
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Pavlovetc, Ilia M.; Podshivaylov, Eduard A.; Frantsuzov, Pavel A.; Hartland, Gregory V.; Kuno, Masaru K. (, Proc. SPIE 11246, Single Molecule Spectroscopy and Super-resolution Imaging XIII)Gregor, Ingo; Erdmann, Rainer; Koberling, Felix (Ed.)Infrared photothermal heterodyne imaging (IR-PHI) represents a convenient table top approach for conducting superresolution imaging and spectroscopy throughout the all-important mid infrared (MIR) spectral region. Although IR-PHI provides label-free, superresolution MIR absorption information, it is not quantitative. In this study, we establish quantitative relationships between observed IR-PHI signals and relevant photothermal parameters of investigated specimens. Specifically, we conduct a size series analysis of different radii polystyrene (PS) beads so as to quantitatively link IR-PHI signal contrast to specimen heat capacity, MIR peak absorption cross-section, and scattering cross-section at IR-PHI’s probe wavelength.more » « less
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